This study on Quito, Ecuador, is part of joint effort of the RUAF Foundation and the Food Business Knowledge Platform, together with local partners, to contribute to a better understanding of how different types of private sector players can shape/enable the existence of a city region food system (as opposed to a more national or international food supply system); and what business and policy environment is needed to better engage the private sector in building such city region food systems. This study focuses on larger processing and retail. Similar studies have been undertaken for Rotterdam, The Netherlands and Bristol, UK. An overall analysis report highlights findings of these three case studies, 19 smaller cases and literature review.